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Fig. 7. Nuclear localisation of Pds5 is cohesin-dependent, and cohesin localisation is altered in the absence of Pds5. (A,B) Fluorescence micrographs showing Pds5-GFP and DNA (Hoechst 33342) localisation in living cells. (A) Left panels show examples from an asynchronous culture of pds5-GFP cells at the cell cycle stages indicated. Right panels show metaphase arrested nda3-KM311 pds5-GFP cells after 12 hours incubation at 18°C. (B) pds5-GFP (rad21+) and rad21-K1 pds5-GFP (rad21-K1) cells grown at 25°C or shifted to 36°C for 3 hours, as indicated. (C) Fluorescence micrographs showing Rad21-GFP and DNA (Hoechst 33342) in living rad21-GFP (pds5+) and pds5{Delta} rad21-GFP cells (pds5{Delta}), as indicated. Bars, 10 µm.