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Fig. 2. [Ca2+]lys and lysosomal pH: resting levels and effects of variable extracellular calcium and bafilomycin A1. (A) [Ca2+]lys. Black bars represent ratiometric measurements using a probe cocktail consisting of furaDx, FDx, and OGDx. The gray bar represents calcium measurements using fluorescence lifetime imaging with the calcium indicator OGBDx. The fluorescence ratio of furaDx was <=Rmin for cells incubated in 10 mM EGTA, therefore [Ca2+]lys was below the detection limit for furaDx at pH 4 (the bar represents the detection limit of furaDx at pH 4). Error bars represent s.e.m. (n>=10 cells). (B) Lysosomal pH. Error bars represent s.e.m. (n>=10 cells).