Fig. 3. A time course showing ratiometric measurements of lysosomal pH () and [Ca²⁺]_lys ([UNK]) following the addition of buffer containing 500 nM bafilomycin A₁ (BAF). Lysosomes were labeled with a probe cocktail containing furaDx, FDx, and OGDx. Arrow indicates time of BAF addition. Error bars represent the s.e.m. (n9 cells).