Fig. 5. Data from a time-course experiment showing the effects of NH₄Cl on macrophage [Ca²⁺]_cyt and [Ca²⁺]_lys. (A) [Ca²⁺]_cyt in activated macrophages ([UNK]) following addition of NH₄Cl and control cells (; activated macrophages without addition of NH₄Cl). (B) [Ca²⁺]_lys ([UNK]) and lysosomal pH () in activated macrophages following addition of NH₄Cl. (C) [Ca²⁺]_lys ([UNK]) and lysosomal pH () in control cells (no NH₄Cl added). To measure [Ca²⁺]_cyt, cells were loaded with FFP-18AM. To measure [Ca²⁺]_lys, cells were loaded with a probe cocktail consisting of furaDx, FDx, and OGDx. Arrows show times of addition of ammonium chloride. Error bars represent s.e.m. (n=30 cells for [Ca²⁺]_cyt measurements and n15 cells for [Ca²⁺]_lys measurements).