Fig. 1. Time course of FBG assembly into the ECM. Confluent human foreskin fibroblasts were incubated with 30 µg/ml Oregon-Green FBG for 1 (A,B), 6 (C,D) or 24 hours (E,F). Cells were washed, fixed and stained with affinity-purified (to remove contaminating antibodies to FBG) rabbit anti-FN IgG, followed by incubation with rhodamine-conjugated goat anti-rabbit IgG (B,D,F). Panels (A,C,E) represent FBG-Oregon-Green fluorescence. Bar in (E) represents 25 µm.