Fig. 2. The effect of serum starvation and LPA on FBG assembly in the ECM of fibroblasts. Confluent monolayers of human foreskin fibroblasts were serum-starved for 24 hours. Medium containing 10% FBS (A,B), no FBS (C,D) or 500 nM LPA (E,F) supplemented with 40 µg/ml FBG-Oregon Green was added to cells and incubated for an additional 18 hours. FBG is shown in (A,C,E), and HSPG staining is shown in (B,D,F). Bar in (F) represents 25 µm.