Fig. 3. FBG assembly into mature matrix fibrils is Rho dependent. Human foreskin fibroblasts were serum starved for 24 hours prior to treatment with 1% FBS-medium with (A) or without (B) PLB (0.1 U/ml) in the presence of FBG-Oregon Green (40 µg/ml). To inhibit RhoA activation, C3 transferase (2 µg/ml) was delivered to serum-starved HFF via LipofectAMINE (10 µg/ml) (C); control cells were treated only with LipofectAMINE (10 µg/ml) (D). FBG-Oregon Green (30 µg/ml) in 1% FBS-medium was added to cells and incubated for an additional 4 hours. Bar in (B) represents 25 µm.