Fig. 7. Effect of MoAb 9D2 on ¹²⁵I-FBG binding to FN^-/- cell layers. Confluent FN^-/- cell layers grown in 48-well plates were incubated for 1 hour at 37°C with 0.2 ml defined medium containing 0.2% BSA, FN (25.8 µg/ml) and ¹²⁵I-FBG (20 µg/ml) in the presence of increasing concentrations of MoAb 9D2 (closed squares) or nonimmune IgG₁ (open circles). After 1 hour of binding, cell monolayers were washed, solubilized in 1N NaOH and radioactivity measured. Binding of ¹²⁵I-FBG is expressed as percentage of binding observed in the absence of 9D2. These data are representative of two experiments each done in triplicate at each IgG₁ concentration. Bars represent±s.e.m.