Fig. 6. Characterization of the valine transport signal. (A) Position dependence of valine. Tails of the indicated sequence were fused to the C-terminus of L53L₁₈R₂ (see Fig. 4). (B) The effect of neighboring amino acids on C-terminal valine-mediated transport of L53L₁₈R₂S₈XX is shown. Acquisition of endo H resistance after pulse-chase was determined as in Fig. 1. Mean values±s.e.m. of at least four independent experiments are shown. An asterisk indicates statistical significance to the SV construct (P<0.05, Student's t-test).