Fig. 5. P63RhoGEF induces RhoA-mediated stress fiber formation in cardiac myoblasts. After 48 hours of transfection, H9C2 cells expressing His-tagged p63RhoGEF (A) or GFP-RhoAV14 (B) were directly fixed and permeabilized as described in Materials and Methods. The expression of p63RhoGEF was detected by the anti-His antibody followed by FITC-coupled anti-mouse immunoglobulins, whereas F-actin was detected by rhodamine-conjugated phalloidin. This experiment is representative of at least three independent experiments.