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Fig. 1. The VID22 gene is necessary for FBPase degradation. (A) Wild-type cells, the vid22-1 transposon mutants and vid22-1 mutants transformed with the VID22 gene were grown in synthetic medium containing low glucose for 2 days to induce FBPase. FBPase degradation was examined after cells were shifted to medium containing 2% glucose for 0, 60 and 120 minutes. (B) FBPase production was induced in wild-type cells, {Delta}vid22 mutants and {Delta}vid22 mutants transformed with the VID22 gene. These cells were shifted to glucose for t=0, 30, 60, 120 and 180 minutes and the degradation of FBPase was examined.