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Fig. 3. VID22 is not required for other vacuolar trafficking pathways. Wild-type, {Delta}pep4, {Delta}agp1 and {Delta}vid22 strains were grown to log phase and examined for the processing of API and CPY. (A) Processing of API in wild-type (lane 1), {Delta}pep4 (lane 2), {Delta}agp1 (lane 3) and {Delta}vid22 (lane 4) strains. (B) Processing of CPY in wild-type (lane 1), {Delta}pep4 (lane 2), {Delta}agp1 (lane 3) and {Delta}vid22 (lane 4) strains. (C) The degradation of peroxisomes in response to glucose was examined using thiolase as a marker protein. Peroxisomes were degraded with similar kinetics in both the wild-type and {Delta}vid22 strains. (D) Wild-type, {Delta}agp1 and {Delta}vid22 strains were incubated for 6 hours in SD(-N) containing 1 mm PMSF to induce the autophagy pathway. The accumulation of autophagic bodies was observed by light microscopy.