Fig. 5. Vid22p sediments in a low-speed fraction. The Vid22p-V5 strain was glucose starved and then shifted to glucose containing media for 30 minutes. Differential centrifugation of the yeast cellular lysate was used to determine the localization of Vid22p (a). The majority of Vid22p was found to localize within the P1 (low-speed) fraction. A small amount of Vid22p was detected in the P13 fraction. The cytosolic marker enolase was found in the S200 fraction (b). The vacuole marker DPAP B and endosomal marker Pep12p were found primarily in the P13 fraction (c-d). The plasma membrane ATPase Pma1p was localized in the P1 fraction (e).