Fig. 6. Vid22p co-localizes with the plasma membrane marker protein, Pma1p. The Vid22p-V5 strain was glucose starved and shifted to fresh glucose media for 30 minutes. Cells were lysed and the total lysate was applied to the top of a 20-50% sucrose gradient. Following a 20 hour spin at 100,000 g, fractions from the sucrose gradient were examined for the distribution of Vid22p (a), the plasma membrane marker Pma1p (b), a COP I vesicle protein Sec21p (c), the Golgi marker Mnn1p (d), the vacuolar protein CPY (e) and the Vid vesicle marker protein Vid24p (f).