Fig. 4. PI3K-independent chemotaxis stimulated by uPA in Dunn chamber chemotaxis assay. (A,B) EGF (15 ng/ml). (C,D) 15 nM mut C uPA. (A,C) Relative cell speeds (basal cell speed=1.0). Positive/negative chemotactic response (+/-) (total number of cells). (B,D) Directionality/displacement of cells stimulated by EGF and uPA±5 µM LY 294002. Mean direction of cell migration (red arrow)/95% confidence interval (green wedge) determined by Rayleigh test. n=total number of cells assayed in three to six experiments. %=proportion of cells that migrate further than 20 µm.