Fig. 7. PI3K-independent and ß₁-integrin dependent activation of Cdc42 and Rac1 by uPA. Cells were stimulated with 10 nM mut C uPA or 10 ng/ml EGF for 10 min±LY 294002 (1-5 µM, pre-treatment 1 hour). (A,B) shows Rac1 activation, and (C,D) shows Cdc42 activation±LY 294002. (B,D) Ratios of GST-PAK-CRIB bound to total Cdc42/Rac1 (band intensity in unstimulated cells=1.0; mean±s.e.m. from 3-7 separate experiments) are shown. (E) Rac1/Cdc42 activation by 10 nM mut C uPA±3 µg/ml anti-ß₁-integrin is shown. (The results are representative of four experiments.)