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Fig. 9. uPA and EGF stimulate N-WASP colocalisation to F-actin with divergent requirement for PI3K activity. (A-F) shows cell staining with Alexa-568-conjugated phalloidin and anti-N-WASP labelled with Alexa-488-conjugated secondary antibody. (A,B) shows unstimulated cells in the (A) absence and (B) presence of LY 294002 for 30 minutes (5 µM; pre-treatment 1 hour). (C,D) The result of a 30 minute stimulation with 10 nM mut C uPA in the (C) absence and (D) presence of LY 294002 is shown. (E,F) 30 minutes stimulation with 10 ng/ml EGF in (E) absence and (F) presence of LY 294002 is shown. Images are representative of three experiments. Bar, 10 µm.