Fig. 4. Effect of vimentin gene inactivation on SGLT1 expression in detergent-resistant membranes (DRM) of proximal tubular cells in primary culture from vimentin-null mice (Vim^-/-; hatched bars) and from wild-type littermates (Vim^+/+; open bars). Cells were solubilized in Triton X-100, DRM were purified on a sucrose flotation gradient and fractions 4-7 were pooled and immunoblotted with a rabbit polyclonal anti-SGLT1 antibody, a rabbit polyclonal anti-5'-nucleotidase antibody and a rabbit polyclonal anti-caveolin antibody. Blots are representative samples from three separate cultures. Data are means±s.e.m. ANOVA: Vim^-/- vs Vim^+/+ cells, ^**P<0.005.