Fig. 6. Effect of methyl-ß-cyclodextrin (MCD) on SGLT1 expression in detergent-resistant membranes (DRM) and brush border membranes (BBM) of proximal tubular cells in primary culture from wild-type mice. Cells were treated or not with MCD (10 mM at 37°C for 2 hours), then DRM (A) and BBM (B) were prepared and analyzed by western blotting using a rabbit polyclonal anti-SGLT1 antibody, a rabbit polyclonal anti-5'-nucleotidase antibody and a rabbit polyclonal anti-caveolin antibody. Blots are representative samples from three separate cultures. Data are means±s.e.m. ANOVA: treated vs untreated cells: P<0.01.