Fig. 8. Vimentin protein localization in proximal tubular cells in primary culture from wild-type animals. (A-C) Cells were fixed in 4% formaldehyde and ice-cold methanol, then incubated with a rabbit polyclonal anti-vimentin antibody (A) and a mouse monoclonal anti-5'-nucleotidase antibody (B), followed by the specific secondary TRITC-and FITC-conjugated antibodies. The overlay is shown in panel C. (D-F) Cells were fixed in 4% formaldehyde and ice-cold methanol, then incubated with a mouse monoclonal antivimentin anti-vimentin antibody (D) and a rabbit polyclonal anti-SGLT1 antibody (E), followed by the specific secondary TRITC- and FITC-conjugated antibodies. The overlay is shown in F.