Fig. 2. Wild-type and mutant forms of Pav-KLP introduced into the Drosophila germ line under UAS control. (A) Different GFP-tagged Pavarotti variants were subcloned into the Drosophila transformation vector pUASp for expression in the female germ line. Regions subjected to site-directed mutagenesis are indicated in yellow boxes. Linking amino acids between the GFP tag and domains of Pavarotti are represented as a blue bar. The first ATG to be translated is given in green. The proteins that derive from these constructs and are used in the text and figures are given below each drawing. (B) Western blots to indicate levels of expression of the indicated constructs in ovaries when driven by GAL4-VP16 under control of the maternal 4-tubulin promoter as described in the text.