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Fig. 4. Localisation of the GFP-tagged stalk domain of Pav-KLP to ring canals. An egg chamber expressing GFP-PavSTALK (green) and stained for Hu-li tai shao (red) and DNA (blue). GFP-PavSTALK appears to be localised at the outer side or ring canals, whereas Hu-li tai shao is more concentrated at the inner side. Regions where the two proteins overlap appear yellow. Bar, 10 µm.