Fig. 5. Stabilisation of cytoplasmic microtubules and disorganisation of nurse cell positioning following overexpression of GFP-tagged PavDEAD or PavNLS(4-7)^*. Live (A,B) or fixed (C-F) egg chambers expressing either GFP-PavNLS5^* (A), GFP-PavNLS(4-7)^* (B,C) or GFP-PavDEAD (D,F) (green). DNA was stained with TOTO-3 (blue, C,D) or Hoechst (E-F). (A) High concentrations of GFP-PavNLS5^* are present in the germ cell nuclei (arrow) and in ring canals (arrowhead). (B) GFP-PavNLS(4-7)^* is excluded from the nurse cell nuclei (arrow) and accumulates in the nurse cell cytoplasm (arrowhead). (C,D) Egg chambers in which nurse cell nuclei (arrowheads) are clustered and surrounded by a dense filamentous network (arrows) associated with GFP-PavNLS(4-7)^* (C) or GFP-PavDEAD (D). (E) A wild-type egg chamber in which the follicular cells have started to migrate to the posterior pole (arrow). The 15 polyploid nurse cell nuclei are all localised outside of the oocyte at the anterior half of the egg chamber (arrowhead). (F) An egg chamber containing mispositioned nurse cells (arrowheads) following GFP-PavDEAD expression. Bars, 20 µm (A,B,D,F) and 50 µm (C,E).