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Fig. 2. Secretion of lysosomal enzymes in lvsA and lvsB mutant cells. Cells were incubated in SB medium to induce secretion of lysosomal enzymes. The activity of three lysosomal enzymes was determined in the cellular pellet and the supernatant at the indicated times. Enzymes tested were {alpha}-mannosidase (A), acid phosphatase (B) and N-acetylglucosaminidase (C). Over the course of the experiment, the total amount of the enzymatic activity did not vary significantly. The percentage of the total activity secreted in the medium is indicated. Filled circles, wild-type cells; open circles, lvsA cells; open triangles, lvsB cells.