Fig. 2. BMP-6 (10 ng/ml), in the presence of laminin-1 (160 µg/ml), promotes cystic colony formation from dissociated E15.5 mouse pancreas cells. Phase contrast images show cells cultured for 2 or 6 days in laminin-1 with and without BMP-6. In the presence of BMP-6, small colonies and some tubule-like structures (closed arrow, also inset) were observed by day 2; by day 6, colonies increased in number and were of variable size. Colonies 30 µm (open arrow) were excluded from the quantitation of colony numbers in Fig. 3 below.