Fig. 4. Effects of TGF-ß superfamily members on pancreatic cell colony formation. Colonies (mean±s.d., n=3 separate experiments) were counted 6 days after culture with the indicated treatment in the presence of 160 µg/ml laminin-1. (A) Effect of BMP-6, activin A, TGF-ß1 (all 100 ng/ml) or vehicle alone. (B) Effect of BMP-5, activin A or TGF-ß1 (each 100 ng/ml) on BMP-6 (10 ng/ml)-induced colonies. (C) Dose-dependent inhibition by TGF-ß1 and activin A of BMP-6 (10 ng/ml)-induced colonies.