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Fig. 6. Characterisation of colony cells. H&E staining showing colonies lined by various types of epithelia (A). Under a higher magnification (oil lens) several types of epithelia are shown: (B) columnar, (C) cuboidal, (D) squamous or (E) a mixture. (F) periodic acid Schiff (PAS) reaction staining of polysaccharides (pink, arrow) in the basement membrane surrounding the colony cells. (G) E-cadherin (E-cad), a marker of epithelial cells, detected by rat anti-mouse E-cadherin antibody and visualised by peroxidase-conjugated rabbit anti-rat immunoglobulins (brown).