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Fig. 7. Immunocytochemistry for proinsulin, insulin, glucagon and amylase in colony cells. The colony cells were directly fixed (for proinsulin only) or harvested for histological sections 6 days after culture. Proinsulin (Pro)-positive cells (also see inset) were stained with monoclonal anti-proinsulin antibody and peroxidase-conjugated rabbit anti-mouse immunoglobulins (A,B). Insulin (Ins)-positive cells were stained with guinea pig anti-insulin serum and visualised by peroxidase-conjugated rabbit anti-guinea pig immunoglobulins. Ins-positive cells constituted and in some cases appeared to bud off from colonies (C-F). Glucagon (Glu)-positive cells (G) and amylase (Amy)-positive cells (H) were stained with rabbit anti-glucagon and anti-{alpha}-amylase antibodies, respectively, and visualized with peroxidase-conjugated swine anti-rabbit immunoglobulins.