Fig. 7. Disruption of syntaxin 1A and CFTR interactions does not alter the
biophysical properties of CFTR; however, open probability increases
significantly. (A) Representative single-channel traces of CFTR in 16HBE14o-
cells prior to and after addition of 350 nM GST-Syn1A
C or 350 nM
GST-Syn1AH3 to the cytoplasmic face of inside-out patches. (B) The
single-channel conductance for currents in the presence or absence of
GST-Syn1AC or GST-Syn1AH3 was 6 pS. The vertical bars are s.e.m.
(n ranged from 1 to 8 at each measured potential). (C) Mean open time
(
o) and mean closed times (c) prior to and
after disruption of syntaxin-1A-CFTR interaction by Syn1AC.
o (
250 mseconds) was unaffected whereas c
decreased from 1.95 seconds to 480 mseconds. (D) Open probability
(Po) in the absence and presence of GST-Syn1AC was
0.03±0.03 and 0.17±0.05, respectively (n=5
(P<0.05)). Po remained unchanged from 0.07±0.03
to 0.04±0.02 following the addition of the negative control fusion
peptide, GST-Syn1AH3 (n=6).