Fig. 6. UV-exposed EGFR was internalised to early endosomes. Cells were either left untreated (A) or exposed to 200 J/m² UV, and incubated for 10 minutes. (B-D). Samples were subject to immunofluorescense confocal microscopy with anti-EGFR (green), and anti-EEA1 to identify early endosomes (red). (A) In unstimulated cells EGFR was located along the plasma membrane. (B-D) Exposure to UV resulted in a redistribution of EGFR to vesicles (B) containing EEA1 (C, and double stained in D). The yellow colour indicates co-localisation. Bar, 10 µm.