Fig. 7. UV-exposed EGFR was arrested in endosomes. (A-C) Cells were exposed to 200 J/m² UV, incubated for 20 minutes, and stained with anti-EGFR. Immunofluorescense confocal microscopy localised the receptor to vesicles (A; red) that were negative for the late endosome marker CD63 (B; green, and double stained in C). (D-F) Cells were exposed to 200 J/m² UV and chased for 60 minutes, with FITC-labelled transferrin present in the last 20 minutes. Staining with anti-EGFR showed a redistribution of the receptor to vesicles (D; red) containing transferrin (E; green, and double stained in F). The yellow colour indicates co-localisation. Representative vesicle aggregates containing EGFR and transferrin are indicated (arrow). Bar, 10 µm.