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Fig. 5. Immunofluorescence of {Delta}504-526 (A-D) and {Delta}512-526 (E-H). Fixed and permeabilized cells were treated with rabbit anti-VSG 117 and fluorescein-conjugated secondary antibody (A,E), with mouse anti-BiP and rhodamine-conjugated secondary antibody (B,F), with DAPI (C,G), and the three images were digitally merged (D, H), which results in a yellow pseudo-color in regions of VSG-BiP colocalization.