(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Effects of elongation mutations on GPI anchoring and expression level. A western blot with rabbit anti-VSG 117, showing the release of VSG117 511FK (A) and VSG117Ty (B). Aliquots representing 2x104 cells were loaded per lane. Lanes C, P1, P2 and S contain, respectively, whole cells, pellet after lysis, and pellet and supernatant after GPIPLC activation. (C) Comparison of VSG expression in four independent clones of VSG117TM, which is not GPI anchored. Aliquots representing 2x104 cells and 2x106 cells were loaded for T7-VSG117 and VSG 117TM, respectively.