Fig. 2. Cells that can be detached following colchicine treatment bind preferentially to Shiga toxin, but bind again to Shiga and Cholera toxin after desynchronization. (a) Vero cells detached from the culture dish after colchicine treatment bind to Cy2-ST (upper left panel). Cells remaining attached to the dish are larger, have an extended structure (arrow) and bind only to Cy3-CTX (lower right panel) but not Cy2-ST (lower left panel). DIC (differential interference contrast) of the two lower panels. (b) Cells detached after colchicine treatment were analyzed 30 minutes after detachment for binding of Cy2-ST (upper left panel) and Cy3-CTX (upper right panel). When detached cells were cultivated for another 12 hours, they show again exclusive binding (arrows) of either Cy2-ST (lower left panel) or Cy3-CTX (lower right panel) as seen in non-synchronized cell cultures (compare with Fig. 1a).