Fig. 1. Protein sequence and alignment of GAPR-1. (A) Peptide sequences determined by microsequencing. (B) cDNA-derived sequence of GAPR-1. The dotted lines indicate the position of the peptides described in (A). The consensus sequence for N-myristoylation is indicated by the box, and the potential protein-protein interacting sites (coiled-coil region and caveolin-interacting region) are marked in grey. (C) Amino-acid sequence alignment of GAPR-1 with relatives of the superfamily. For each of the subfamilies, including human GliPR, CRISPs, plant PR-1 proteins, allergens of insect venoms and snake or lizard venoms, the protein sequence of the member with the highest homology to GAPR-1 is aligned to GAPR-1. Other sequences shown are (with Swiss Prot accession numbers between brackets) Drosophila CG2337 (AE003674), pathogenesis-related protein PR1 (U64806), hypothetical 30.6 kDa protein in yeast PRY1 (P47032), venom allergen 5 AG5 (P35784), testis-specific protein TPX1 (P16562) and Glioma pathogenesis-related protein GliPR (P48060). Identical amino-acid residues in family members are highlighted in black. Conservative amino-acid exchanges are highlighted in grey.