Fig. 5. Immunolocalization of GAPR-1 to the Golgi complex. Vero cells were incubated in the absence (top panels) or presence of BFA (5mM) for the indicated times (5-30 minutes) and processed for double immunofluorescence. Left and middle panels represent immunofluorescent labelling of the cells with an antibody against GAPR-1 and the KDEL receptor, respectively. The right panels shows the colocalization of GAPR-1 and KDEL receptor by merging the two panels. Bar, 20µm.