Fig. 6. Colocalization of GAPR-1 with anterograde cargo in the intermediate compartment. Vero cells were infected with vesicular stomatitis virus tsO45 (VSV-G tsO45) expressing a glycoprotein with temperature-sensitive folding properties. After infection, the cells were incubated at the non-permissive temperature of 39.5°C (upper panels). Two representative examples are shown of the subsequent incubation of the cells at 15°C (middle and lower panels). The cells were analysed for the localization of GAPR-1 and VSV-G by double immunofluorescence as described in the Materials and Methods. Bar, 20 µm.