Fig. 4. (A) MMPs regulate lung bud branching in lung organ cultures. Numbers above
the panels represent the number of days in culture. E11.5 lung buds were
isolated from timed-pregnancies and placed in serum-free media in the presence
or absence of MMP inhibitor (GM6001). Following four days growth in vitro,
lung buds show retardation of branching in the presence of MMP inhibitor as
compared to control. (B) Analysis of branching inhibition. Quantification by
counting the number of newly formed secondary and tertiary branches in E10.5
(n=4) and E11.5 (n=3) whole lungs following four days of
culture. *P
0.05 (Student's t-test).