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Fig. 5. HBP is required for histone gene expression. (A) Extracts prepared from wild-type and R06F6.1(RNAi) C. elegans were analysed on a 15% SDS-PAGE. Proteins were detected by western blotting using antibodies against histone H3 (lanes 1, 2) or histone H4 (3, 4), as well as with an anti-tubulin antibody to control for loading. (B,C) Epifluorescence image showing immunostaining with anti-histone H3 antibody of a wild-type (B) and an R06F6.1(RNAi) embryo (C). Scale bars represent 10 µm.