Fig. 5. Distribution of ß1-integrin in the absence and presence of laminin. Cells were incubated with 2 µg/ml of laminin for 8 hours. Cells were fixed, immunostained with laminin-specific antibody, then permeabilized with Triton X-100 and co-stained with anti-ß1 integrin antibody. (a) ß1-integrin was distributed in fibrillar arrays prior to treatment with laminin. Panels b and c reveal integrin and laminin within the same field. Following laminin incubation, co-localization on a subset of integrin fibrils with laminin (arrows indicate examples) was observed. Bar, 10 µm.