Fig. 6. Distributions of cytoskeletal components. Laminin at 5 mg/ml was incubated with Schwann cells for 18 hours. Effect of laminin cell surface assembly on several cytoskeletal proteins was evaluated. (a-c) The distribution of utrophin was detected with monoclonal antibody MANCHO3 with and without laminin treatment. Utrophin reorganization was noted with laminin treatment (compare a and b, border shown with arrows). Laminin-utrophin co-localizations were noted after 18 hours of laminin incubation. (d-g) Vinculin (Vn; d,e) and paxillin (Pax; f,g) were noted to be present on the cell/plastic interface; neither co-localized with a laminin matrix. Bar, 10 µm