Fig. 8. Contributions of type IV collagen. EHS type IV collagen was incubated with Schwann cells for 4 hours with or without laminin. Cells were washed, fixed and double-stained with anti-laminin (a,c,e) and anti-collagen type IV (b,d,f) antibodies and corresponding Cy3-or FITC-conjugated secondary antibodies. Accumulation of exogenous laminin on the cell surface occurred without the presence of type IV collagen (a). Exogenous type IV collagen accumulated on the cell surface (d,f), even in the absence of laminin (d), forming mostly fibrillar structures (arrowheads). In the presence of laminin, exogenous type IV collagen also assembled into the reticular type of pattern (f, arrows, compare laminin in e). Bar, 10 µm.