Fig. 11. Editing activity and the proportion of total cellular p66/ACF in the nucleus are enhanced following ethanol and insulin treatment. RNA was extracted from cultures of rat primary hepatocytes treated with ethanol or insulin for 6 hours. Editing activity was quantified by the poisoned primer extension assay, whereas cultures treated in parallel were subfractionated into cytoplasm proteins and nuclear proteins and assayed for p66/ACF by western blotting. Editing activity is shown as the average of three experiments ± s.e.m. The relevant regions of western blots for cytoplasmic proteins (C) and nuclear proteins (N) are shown and were prepared as described in Fig. 5. The nuclear to cytoplasmic (N/C) ratio was determined as described in Fig. 5 and did not vary more than 10% within treatment groups in the three replicate experiments.