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Fig. 4. Expression of P66/ACF mRNA and protein. (A) A northern blot of total cellular rat polyA+ mRNA from the indicated tissues was probed with p66/ACF cDNA and autoradiographed. The relative migration of size markers is shown to the left. (B) A western blot of total cellular protein from the indicated cell lines was reacted with peptide-specific polyclonal antibodies and visualized by chemiluminescence and autoradiography.