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Fig. 7. Immunohistochemical localization of rat liver p66/ACF. Rat liver sections were prepared and subjected to immunocytochemistry using anti-p66/ACF. (A) Low power (20x) image of a liver section showing the distribution of central veins with associated dense p66 reactivity. (B) Higher power (40x) images of two of the central veins regions in A showing regionally high concentrations of p66/ACF reactivity. Individual hepatocytes were made apparent by nuclear staining with hematoxylin counter staining.