Fig. 1. Effect of forskolin on DNA synthesis. (A) Reh cells (0.1x10⁵ cells/ml) were treated with forskolin (100 µM) for 3 days and DNA synthesis was measured as uptake of [³H]thymidine, as described in Materials and Methods. Vertical bars indicate the s.e.m. of three experiments. (B) Reh cells (0.1x10⁶ cells/ml), with or without forskolin (100 µM) treatment, were counted for 10 days and the cell counts are presented as fold induction of the starting culture (0.1x10⁶ cells/ml). One representative experiment is shown. Inset shows the cell counts up to 4 days with or without forskolin (100 µM) treatment. The cell counts are presented as the mean from five separate experiments. The vertical bars represent standard error of the mean (s.e.m.). C, control; F, forskolin.