Fig. 5. Inhibition of katanin by CFP-P loop K-A p60 reduces the rate of nocodazole-mediated spindle disassembly. CV-1 cells with an integrated YFP--tubulin transgene were transiently transfected with CFP-P loop K-A p60 and mounted in a perfusion chamber. Transfected cells were identified by CFP fluorescence (A) and spindle disassembly was monitored by shuttered, time-lapse imaging of YFP-fluorescence (B-F) after perfusion with nocodazole. Note that the spindle in the untransfected cell (bottom right) shortened and disappeared rapidly, whereas the spindle in the CFP-P loop K-A p60-expressing cell (top left) shortened and faded much more slowly.