Fig. 6. F-actin structures in nda3 mutant cells. nda3 mutant cells were incubated at 20°C for 8 hours to arrest. For release experiment, once arrested, cells were re-incubated at 36°C and collected every 2 minutes. These cells were immediately fixed at each time, and then stained simultaneously with DAPI and Bodipyphallacidin. (a-h) Photographs obtained by conventional fluorescence microscopy. Left, DAPI stainings; right, Bodipy-phallacidin stainings. (a,b) Arrested cells. (c-h) Released cells: 4 minutes (c,d), 10 minutes (e,f) and 14 minutes (g,h) after temperature shift-up. (i,j) 3D images. Green and blue represent F-actin and DNA, respectively. (i) Arrested cells. (j) Cells at 4 minutes after the release. Arrows indicate accumulated F-actin cables. Bar, 2 µm.