Fig. 6. Quantitation of the effects of Rab22a on rho-EGF degradation in Hep2 cells. Hep2 cells were transfected with pGEM-myc, -mycRab22a wt, -mycRab22a Q64L or -mycRab22a S19N using the Vaccinia system. The cells were incubated with rhodamine-labelled EGF for 1 hour at 37°C, followed by a 3 hour chase at 37°C before fixation and staining with an anti-myc antibody. Transfected cells were visualized by confocal immunofluorescence microscopy, and 150 cells on separate coverslips were counted for the presence of rho-EGF signal after a 3 hour chase. The data are presented as the % (±s.e.m.) of transfected cells having a visible rho-EGF signal after the chase.