Fig. 1. Localisation of Asp protein in mitosis and meiosis during spermatogenesis. The merged image (left hand panels) shows DNA (blue), microtubules (green) and Asp (red). The channel showing Asp protein alone is shown in the central panels and microtubules alone in the right hand panels. (A) A four-cell cyst at metaphase and (B) an eight-cell cysts during late telophase; note the Asp ring between daughter nuclei (arrow). Progression through the first meiotic division: (C) Prometaphase meiotic spindles with Asp at the poles. (D) Metaphase meiotic cells with Asp staining strongly at the spindle poles and weakly in the midpoint of the spindles (arrowheads). (E) Telophase cells of meiosis I in which the Asp at the spindle poles falls into two clusters around the centrioles that are beginning to separate. In addition, these cells show prominent Asp staining along the putative minus ends of the central spindle microtubules, which are shown at higher magnification in (F) (arrowhead). The arrow in (E) and (F) point to a very central band of Asp staining that can also be seen in the central spindle. Scale bar is 10 µm.